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The human ORFeome collection is made possible through support from the High-Tech Fund of the Dana-Farber Cancer Institute, The Ellison Foundation (Boston, MA), and by grants from the National Cancer Institute, the National Human Genome Research Institute, and the National Institute of General Medical Sciences.

About Human ORFeome

The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs) collected into ORFeome collections, so as to allow functional studies of the corresponding proteomes. Here we present a fourth version of the human ORFeome, human ORFeome 7.1.

With the Mammalian Gene Collection (MGC) resource as a starting point, we successfully cloned 18,414 human ORFs, representing at least 15,415 human genes, as mini-pools of PCR-amplified products. The human ORFeome version 7.1 (hORFeome v7.1) collection, includes the previous human ORFeome releases (v1.1, v3.1 and v5.1), with the addition of 2,231 newly transferred ORFs from MGC templates.

Human ORFeome Statistics

  • ORFs cloned: 18,414

  • Non-redundant human genes: 15,415