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The human ORFeome collection is made possible through support from the High-Tech Fund of the Dana-Farber Cancer Institute, The Ellison Foundation (Boston, MA), and by grants from the National Cancer Institute, the National Human Genome Research Institute, and the National Institute of General Medical Sciences.

About Human ORFeome

The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs) collected into ORFeome collections, so as to allow functional studies of the corresponding proteomes. We present a new version of the human ORFeome, human ORFeome 8.1 containing clonally-derived and sequence-confirmed ORFs as a set of Gateway Entry clones ready for transfer to Gateway-compatible expression vectors.

With the Mammalian Gene Collection (MGC) resource as our original starting point, we obtained 12,230 clonal isolates, representing at least 11,149 human genes, from our previous collection of human ORFs originally cloned as mini-pools of PCR-amplified products. Next-generation sequencing was employed to derive human ORFeome version 8.1 (hORFeome v8.1), which encompasses all previous human ORFeome releases.

hORFeome v8.1 represents a central resource of single-colony, fully-sequenced cloned human ORFs which can be readily transferred to Gateway compatible destination vectors for various functional proteomics studies. This set of ORFs ranges in size from 75 to more than 10,000 base pairs, and contains over 1,000 ORFs from genes with multiple splice variants.